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LIVE/DEAD stain of SMCs cocultured with Silk MPs and SIMPs incubated in PBS or digested with Protease XIV showed low cytotoxicity of the particles. SMCs were also cultured with water as a negative control. Green channels (a,c,e, and g) showed confluent live cells in all MP treated groups compared to the negative control (i). Red channels (b,d,f, and h) showed minimal amounts of red (dead) cells compared to the negative control (j). The number of live (green) and dead cells (red) was counted across treatments ( n = 3), and the percentage of live and dead cells was compared to the negative control. There was a significantly higher percentage of live cells (k) and simultaneously a significantly lower percentage of dead cells (l) in the MP groups compared to the negative control. Scale bar = 100 μm ** = p < 0.01, *** = p < 0.001

Journal: ACS Applied Materials & Interfaces

Article Title: Chemical Conjugation of Iron Oxide Nanoparticles for the Development of Magnetically Directable Silk Particles

doi: 10.1021/acsami.4c17536

Figure Lengend Snippet: LIVE/DEAD stain of SMCs cocultured with Silk MPs and SIMPs incubated in PBS or digested with Protease XIV showed low cytotoxicity of the particles. SMCs were also cultured with water as a negative control. Green channels (a,c,e, and g) showed confluent live cells in all MP treated groups compared to the negative control (i). Red channels (b,d,f, and h) showed minimal amounts of red (dead) cells compared to the negative control (j). The number of live (green) and dead cells (red) was counted across treatments ( n = 3), and the percentage of live and dead cells was compared to the negative control. There was a significantly higher percentage of live cells (k) and simultaneously a significantly lower percentage of dead cells (l) in the MP groups compared to the negative control. Scale bar = 100 μm ** = p < 0.01, *** = p < 0.001

Article Snippet: Human aortic vascular smooth muscle cells (SMCs) from Cell Applications (354–05a, San Diego, CA) were cultured at 37 °C and 5% CO 2 (3530, Isotemp, ThermoFisher Scientific) in supplemented basal media (311 K-500, Cell Applications Inc.) between passages 6–10.

Techniques: Staining, Incubation, Cell Culture, Negative Control

ArtMSCs did not induce SMC toxicity. ArtMSCs and Blank-MPs were incubated with SMCs for 6, 12, and 24 h, after which the SMCs were incubated with a fluorescent LIVE/DEAD assay. Green cells indicate live cells while red cells indicate dead ones. Blank-MPs caused some cytotoxicity at 12 h and 24 h, as indicated by the red cells shown by the orange arrows. The cell death control (Dead NC using diH 2 O) and live cell control (Live PC using SBM) both indicate that the LIVE/DEAD stain was functional.

Journal: Bioengineering

Article Title: Mesenchymal Stem Cell-Conditioned Media-Loaded Microparticles Enhance Acute Patency in Silk-Based Vascular Grafts

doi: 10.3390/bioengineering11090947

Figure Lengend Snippet: ArtMSCs did not induce SMC toxicity. ArtMSCs and Blank-MPs were incubated with SMCs for 6, 12, and 24 h, after which the SMCs were incubated with a fluorescent LIVE/DEAD assay. Green cells indicate live cells while red cells indicate dead ones. Blank-MPs caused some cytotoxicity at 12 h and 24 h, as indicated by the red cells shown by the orange arrows. The cell death control (Dead NC using diH 2 O) and live cell control (Live PC using SBM) both indicate that the LIVE/DEAD stain was functional.

Article Snippet: Human aortic vascular smooth muscle cells (SMCs) either from ATCC (for toxicity assessment, Manassas, VA, PCS-100-012) or Cell Applications (for proliferation assays, 354-05a, San Diego, CA, USA) were cultured in supplemented basal media (SBM) (311K-500, Cell Applications Inc.).

Techniques: Incubation, Live Dead Assay, Control, Staining, Functional Assay